A simple, precise, accurate, reproducible RP-HPLC method was developed and validated for the simultaneous estimation of Clonazepam and Propranolol hydrochloride in bulk and in pharmaceutical dosage forms. Chromatographic separation was carried out on a Thermo-hypersil C8 column (250mm×4.6mm i.d,5µm) utilizing a mobile phase consisting of acetonitrile and 0.01M ammonium phosphate buffer (pH adjusted to 3.0 with ortho phosphoric acid) in the ratio of 60:40 v/v at a flow rate of 1ml/min with UV detection at 238nm. The retention times of Clonazepam and Propranolol hydrochloride were 3.362 min. and 2.524 min. respectively. The developed method was validated in terms of accuracy, precision, linearity, specificity, limit of detection and limit of quantification. The linear range was found to be 0.5-3.0µg/ml and 20-120µg/ml for Clonazepam and Propranolol hydrochloride respectively. The proposed method can be used for estimation of these drugs in combined dosage forms.
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